污污网站观看-污污网站大全-污污视频在线免费看-污污视频在线观看黄-91精品日韩-91精品人成在线观看

您的位置:首頁 > 技術支持 > 超濾管使用說明

超濾管使用說明

更新時間:2017-11-10      4471

Introduction 
Amicon® Ultra-15 10K centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. The  vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Typical processing time is 15 to 40 minutes. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to  dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipettor, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging-bucket or fixed-angle rotor. Amicon® Ultra-15 10K devices are supplied non-sterile and are for single use only.
 Intended Use 
The Amicon® Ultra-15 product line includes 5 different cutoffs (Molecular Weight Cutoff, MWCO), however, the Amicon® Ultra-15 10K device (10,000 MWCO) is the only device intended for in vitro diagnostic use. It can be used to concentrate serum, urine, cerebrospinal fluid, and other body fluids prior to analysis. For information on other Amicon® Ultra-15 cutoffs, go to www.millipore。。com and enter Amicon Ultra-15in the search box.
Applications 
 ●Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids  (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and  purified samples 
●Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal prior to HPLC 
●Desalting, buffer exchange, or diafiltration 
Materials Supplied 
The Amicon® Ultra-15 10K device is supplied with a cap, a filter device, and a centrifuge tube
Required Equipment  
●Centrifuge with swinging-bucket or fixed-angle rotor with wells/carriers that can accommodate  50 mL tubes
CAUTION: To avoid damage to the device during centrifugation, check clearance before spinning. 
●Pipettor with 200 microliter (μL) tip for concentrate recovery 
Suitability 
Preliminary recovery and retention studies are suggested to ensure suitability for intended use. See the “How to Quantify Recoveries” section. 
Shelf Life 
Shelf life is 3 years from date of manufacture. Refer to expiration date on package label.
Rinsing Before Use 
The ultrafiltration membranes in Amicon® Ultra-15 10K devices contain trace amounts of glycerine. If this material interferes with analysis, rinse the device with buffer or Milli-Q® water before use. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water.  
CAUTION: Do not allow the membrane in Amicon® Ultra filter devices to dry out once wet. If you are not  using the device immediay after rinsing, leave fluid on the membrane until the device is used. 
How to Use Amicon® Ultra-15 Centrifugal Filter Devices 
1. Add up to 15 mL of sample (12 mL if using a fixed-angle rotor) to the Amicon® Ultra filter device.
2. Place capped filter device into centrifuge rotor; counterbalance with a similar device. 
3. When using a swinging-bucket rotor, spin the device at 4,000 × g maximum for approximay 15–40 minutes.When using a fixed-angle rotor, orient the device with the membrane panel facing up and spin at 5,000 × g maximum for approximay 15–40 minutes.NOTE: Refer to Figure 1 and Table 1 for typical spin times. 
4. To recover the concentrated solute, insert a pipettor into the bottom of the filter device and withdraw the sample using a side-to-side sweeping motion to ensure total recovery. The ultrafiltrate can be stored in the centrifuge tube.
NOTE: For optimal recovery, remove concentrated sample immediay after centrifugation.
 Desalting or Diafiltration 
Desalting, buffer exchange, or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon® Ultra-15 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any  desired solvent. The process of “washing out” can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below

點擊這里給我發消息
點擊這里給我發消息
 
主站蜘蛛池模板: 一97日本道伊人久久综合影院 | 狠狠综合久久久久综 | 成人欧美精品一区二区不卡 | 精品久久中文久久久 | 一级片美女| 国产成人亚洲精品91专区高清 | 看真人视频一级毛片 | 亚洲国产成人久久一区www | 亚洲一区二区三区久久久久 | 夜夜操综合 | 福利午夜国产网站在线不卡 | 欧洲成人全免费视频网站 | 网站视频在线观看 | 亚洲第一永久在线观看 | 欧美aaa级片| 亚洲精品第一国产综合高清 | 四虎最新免费观看网址 | 免费网站看v片在线成人国产系列 | 国产专区在线播放 | 国产精品视频免费播放 | 香蕉521av网站永久地址 | 悠悠影院欧美日韩国产 | 久久免费精品国产72精品剧情 | 日韩 亚洲 制服 欧美 综合 | 深夜免费视频 | 爱爱黄色| 欧美国产精品不卡在线观看 | 久草综合视频 | 国产精品9999 | 福利资源在线观看 | 毛片网站有哪些 | 亚洲国产精品综合久久 | 国产欧美亚洲精品a | 美国特级毛片 | 欧美综合国产精品日韩一 | 欧美精品一二区 | 五月天com | 四虎影视永久免费观看地址 | 九九久久九九 | 色久优优 | 欧美精品一区二区三区四区 |