PIERCE，LC-SMCC: Succinimidyl-4-（N-Maleimidomethyl
- 型號： 22362
- 更新時間： 2024-05-31
- 上一個：22360PIERCE，（SMCC） Succinimidyl 4-（N-Maleimidomethyl）Cy
- 下一個：22363PIERCE，SMPH: Succinimidyl-6-（（B-maleimidopropiona
訂貨：，，
LC-SMCC: Succinimidyl-4-（N-Maleimidomethyl）Cyclohexane-1-Ca介紹
Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
LC-SMCC: Succinimidyl-4-（N-Maleimidomethyl）Cyclohexane-1-Ca現貨供應
PIERCE | 22362 | LC-SMCC: Succinimidyl-4-（N-Maleimidomethyl）Cyclohexane-1-Ca | 50 MG |
PIERCE | 22363 | SMPH: Succinimidyl-6-（（B-maleimidopropionamido） hexanoate, | 50 MG |
PIERCE | 22416 | SUCCINIMIDYL 4-（P-MALEIMIDOPHENYL）BUTYRATE | 50 MG |
LC-SMCC: Succinimidyl-4-（N-Maleimidomethyl）Cyclohexane-1-Ca品牌介紹
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LC-SMCC: Succinimidyl-4-（N-Maleimidomethyl）Cyclohexane-1-Ca
僅用于科研
Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm （16.2 angstroms）.
LC-SMCC is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of LC-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.
LC-SMCC is often used for the preparation of antibody-enzyme and hapten-carrier conjugates. In this type of conjugation, the NHS ester is reacted first with the antibody, excess crosslinking reagent removed and then the sulfhydryl-containing enzyme molecule is added. This two-step reaction scheme results in formation of specific antibody-enzyme conjugates.
LC-SMCC is a reagent of choice for creating stable maleimide-activated carrier proteins that will spontaneously react with sulfhydryls. In this application, the NHS ester of LC-SMCC is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. These relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.
Highlights:
- Amine reactive NHS ester crosslinks rapidly with primary amine-containing molecule
- Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
- High purity, crystalline LC-SMCC can be used to create high-purity maleimide-activated derivatives
- Cyclohexane bridge confers added stability to the maleimide group making LC-SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C。
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