PIERCE,Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg m
- 型號： 22622
- 更新時間： 2024-05-31
- 上一個：22589PIERCE，（SULFO-SANPAH） SULFOSUCCINIMIDYL 6-（4'-AZID
- 下一個：22980PIERCE,1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMI
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Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg microtubes介紹
Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg microtubes現貨供應
PIERCE | 22622 | Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg microtubes | 8 X 2 MG |
PIERCE | 22980 | 1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCl （EDC） | 5 GM |
PIERCE | 22981 | 1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL （EDC） | 25 GM |
Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg microtubes品牌介紹
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Sulfo-SMCC, No-Weigh（tm） Format, 8 x 2 mg microtubes僅用于科研
Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.
Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Sulfo-SMCC Highlights
- Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
- Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
- Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
- High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
- Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.
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