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ProFound Mammalian c-MycTag IP/Co-IP Kit介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

ProFound Mammalian c-MycTag IP/Co-IP Kit現(xiàn)貨供應(yīng)

ProFound Mammalian c-MycTag IP/Co-IP Kit品牌介紹

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1）化學(xué)發(fā)光體和比色底物

2）分析試劑

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ProFound Mammalian c-MycTag IP/Co-IP Kit僅用于科研

The Thermo Scientific Pierce c-Myc Tag IP/Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation （IP） or co-immunoprecipitation （co-IP） experiments using a c-Myc-tagged protein as the "bait".

The c-Myc peptide （EQKLISEEDL） has become a popular fusion tag for mammalian recombinant protein expression. This kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized. Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged "bait" protein are captured on the agarose beads. After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis. The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions.

Highlights:

• Specific – the immobilized anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection （Figures 1 and 2）
• High Capacity – excellent results with as little as 1 µg of anti-c-Myc antibody in IP mode with the positive control lysate
• Rigorous – kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls （see Figure 2 for example controls）
• Robust – the affinity system is compatible with IP or Co-IP from various cell lysates and physiological （non-denaturing） buffer systems; choose the Mammalian Kit to use the validated M-PER Mammalian Cell Lysis Reagent for the procedure
• Convenient and Easy – complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions