重組人骨形態發生蛋白（rHuBMP-4）
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- 更新時間： 2024-05-23
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產品介紹
| CYT-361 | 重組人骨形態發生蛋白（rHuBMP-4） | 2μg/10μg/1mg | Prospec |
| Background : | |
 | Description : |
| Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| BMP-4 was lyophilized from a concentrated （1mg/ml） sterile solution containing no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Applications: |
1.Molecular standard （Western, ELISA） in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Bone Morphogenetic protein-4 . | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm 2. Analysis by RP-HPLC, using a standard solution of BMP-4 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國光譜醫學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！
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