重組人可溶性CD4 （rsCD4）
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- 更新時間： 2024-05-23
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產品介紹
| CYT-304 | 重組人可溶性CD4 （rsCD4） | 2μg/10μg/1mg | Prospec |
| Background: | |
| CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC （Major Histocompatibility Complex） Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 （+）and CD8 （ - ）produce factors such as IL-4 , IL-5 ,IL-10 and IFN-gamma | |
 | Description : |
| Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 40,000 Dalton. As a result of glycosilation the sCD4 migrates as a 46,000 dalton protein on standard SDS-PAGE. Detected By Western Imunno Blots anti cd4 monoclonal antibody. Recombinant soluble CD4 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered colorless liquid formulation. | |
| Formulation: |
| Recombinant Human Soluble CD-4 solution contains 10mM sodium phosphate buffer pH-7.2, 300mM sodium chloride and 0.01% tween. | |
| Stability: |
| rHusCD4 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 90.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Recombinant Human Soluble CD-4 is fully biologically active when compared to standard. Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of soluble CD4. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of CD4 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國光譜醫學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！
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